実験的肝炎“ヒト伝染性肝炎患者胆汁接種による実験的家兎肝炎”
1974 年 34 巻 2 号 p. 181-189
詳細
1974 年 34 巻 2 号 p. 181-189
A fulminant hepatitis was experimentally induced in 5 out of 20 rabbits by inoculation with duodenal juice, obtained from patients with infectious hepatitis, by the combination of daily injections of steroid hormone and insulin.
Histologic findings were characterized by multifocal liver necrosis surrounding by infiltration of a great number of polymorphonuclear lemocytes and mononucler cells. These findings were observed during the period between 5 and 12 days after injection of duodenal juice. The pathological findings were closely similar to acute yellow atrophy of the liver of human.
Rabbits, used as a control, which were injected only steroid-hormones and insulin showed no abnormality by the light microscopy.
Laboratory test, including S-GOT and S-GPT, showed no significant difference between experiment and control animals, although S-GOT of a rabbit rised slightly after injection of duodenal juice.
Immunolo-fluorescent study, using F.I.T.C. (Fluorescein isothiocyanate) labeled rabbit antibody against Australian antigen, showed no specific staining in the liver tissue at all.
