Investigation for drug dependence using in brain vivo microdialysis

Abstract

We used in vivo microdialysis methods to clarify the mechanism of a drug dependence-related novel molecule Shati/Nat8L, and the therapeutic tools for drug dependence Leu-Ile and Pseudoginsenoside-F11 (PF11). Overexpression of Shati/Nat8L in the mice nuclear accubems was rescued the methamphetamine induced place preference and sensitization. In vivo microdialysis experiments showed that Shati/Nat8L reduced the DA release from nuclear accubems in mice. Further, Leu-Ile has therapeutic actions in the methamphetamine-treated-mice. Interestingly, repeated administration of Leu-Ile suppressed dopamine release induced by methamphetamine in mice, although one shot administration of Leu-Ile has no effect on the dopamine release. PF11 is an ocotillol-type saponin that is isolated from American ginseng and shown protective effects against methamphetamine preference and dopamine release. In vivo microdialysis methods also showed that GABA regulates the DA release in mice. Taken together with these results, In vivo microdialysis methods are essential for investigation of the mechanism of drug dependence.