The nature of biomaterial surface influences bacterial adhesion. Adherence of bacteria to biomaterials is the first stage in the development of a device related infection. The ability of bacterial cells to attach to biomaterial surfaces is dependent on the physico-chemical characteristics of the bacterial cell, the bacterial growth conditions and the biomaterial surface chemistry. In the present work an attempt was made to investigate the different growth media on the surface properties of bacteria isolated from indwelling urinary catheters and on their ability to adhere to polyurethane.The role of biomaterial conditioning film formation on the adherence of the isolates to polyurethane and on the polymer surface characteristics were also examined.