In order to achieve more perfect vibration-free environment in base-isolated semiconductor factories, a smart structure was tested for comprehensive active microvibration control in a base-isolated 2-story steel frame building model of a 5×3×4H m external size and a 6,900 kg total mass which was supported by four multistage rubber bearings. In the building model, 20 piezoelectric actuators were attached to the columns and the beams, and eight actuator units consisting of two piezoelectric actuators each were installed between the bottom of the superstructure and the foundation. The controller was designed by a model-matching method. The tests showed that the smart structure could effectively control three-dimensional both microvibrations generated by equipments and people in the buildings and excited by external disturbances such as ambient ground vibrations and winds.