Abstract
Deformation of actin fibers in a living osteoblast under applied shear deformation has been visualized by green fluorescent protein transfection. We obtained displacement of each position of actin fibers by a manual method and KLT method. Comparing two results, we confirmed that KLT method is able to obtain deformation of actin fibers in a cell. Actin fibers were moved in the direction of the tension of actin fibers when a part of actin fibers is broken. This result indicates that static equilibrium with actin fiber's tension play an important role in mechanical structure of a cell.
