3次元ゲル包埋培養ヒト神経細胞の分化・成長に及ぼす動的力学刺激の影響

抄録

Human iPS cells were established in 2007, and researches on regenerative medicine using the cells have been conducted around the world. In 2018, clinical studies are planned for transplanting myocardial sheets prepared from iPS cells to heart failure patients. First, it is important to create 3-D structures from iPS cells. There are many tasks. For example, it takes a huge cost to cultivate cells, and it takes a long time to differentiate iPS cells into target cells. On the other hand, cells in the body are subjected to various mechanical stimulation in vivo, and these are thought to affect cell growth. In this study, we cultured the cells in the 3-D gel embedded culture in the differentiation process from iPS cells to neurons, and applied dynamic stimulations. We examined the effects of the differentiation and growth of cells, directionality and extension rate of neurites. To apply dynamic stimulation, we used a vibration stage. Vibration conditions are set us the frequency: 1, 5, 10, 15, 20Hz, the amplitude: 60μm, and the incubation time: 48hours, horizontal direction. As a result, it was suggested that when neurons are applied dynamic stimulation, in all frequency range differentiation and growth rate exceeds static culture. Especially, 10 Hz stimulation case indicated the highest increase rate against the static and significant differences were observed. Regarding to the directionality of the neurites, when neuron is applied dynamic stimulation, there was a tendency to extend in the direction perpendicular to the vibration direction. Regarding to the extensibility of the neurites, there was a tendency to decrease in proportion to frequency increase of the dynamic stimulation.