マウス神経細胞の簡易移植システム構築及びそれに対する動的力学刺激の影響

抄録

In this study, the simulated simple transplantation system of the neurons differentiated form of mouse iPS (induced pluripotent stem) cells is investigated. Mouse iPS cells are differentiated into neurons by SFEBq method. Two colonies of neuronal cells are prepared, one is the recipient cells and the other is donor cells. At first, suspension culture of recipient cell is started. Then, in 5 days, suspension culture of donor cell is started with recipient cell shifted to adhesion culture. In 10 days, transplantation of donor cell is conducted next to recipient cell. Neuron networks between the recipient cells and the donor cells are jointed for 5 days of culturing. Then, the dynamic stimulation is applied using the 3-D vibration stage to the neuronal networks to promote formation and merging. The total stimulation time is set as 96 hours, that is the vibratory stimulations are applied to the transplantation system during 4 days from one day after transplantation. As a result, it is suggested that the neuron networks are constructed actively with the dynamic stimulation.