培養軟骨細胞を用いた再生組織形成に対する機械的振動の影響

抄録

It has been reported that cells show adaptive response by sensing mechanical vibration. The mechanism of it is not clear but this phenomenon can be applied to regenerative medicines of articular cartilages. In this study, chondrocytes from porcine articular cartilages were cultured without scaffolds. Control groups and vibration groups were prepared for comparison and they were incubated for 20 days in order to make cell sheets of chondrocytes. The condition of mechanical vibration was sinusoidal wave of 12.5 Hz and 0.5 G. To observe the effect on production of cartilage matrices and cell proliferation, immunohistochemical examination with staining proteoglycan was conducted and the numbers of cells were measured in order to control groups and vibration groups be compared. As a result, thickness of chondrocyte cell sheets was increased by mechanical vibration at 12.5 Hz and 0.5 G, but significant difference was not shown. From the result of measurement of the number of cells, there were no significant differences between control groups and vibration groups.