細胞接着力の超音波測定法構築のためのマイクロ流路の開発

抄録

Cell adhesion is closely related to various cellular properties. However, there is no effective method to measure cell adhesion. Meanwhile, we have developed an enzyme-free detachment method, which can detach cells by irradiating ultrasonic. Hence, we set out to develop a method for measuring the adhesive force of cells by irradiating ultrasonic from the bottom of the dish. As a preliminary experiment for this purpose, we fabricated a cell detachment microfluidic device for irradiation of ultrasonic on a cell in order to focus on observation of one cell. The microchannel was made of a cover glass and PDMS, and the ultrasonic vibrator was fabricated by using a transducer with a glass plate and a piezoelectric ceramic plate. By using this device, the mesenchymal stem cells were cultured in the microchannel, and ultrasonic irradiated to a single cell cultured in the microchannel. By increasing the output voltage of the transducer, cells detached by the ultrasonic were observed.