損傷ヒト神経細胞コロニーの再構築に及ぼす動的力学刺激の影響

抄録

Neuronal cells, which are generally said to be hard to recover from physcical damage, are equipped with function of a sensor that senses stimuli and extends axons has been elucidated. In this study, we investigated the effect of the dynamic stimulation on damaged human neuron colonies in neuronal network reconstruction. The neuronal cells differentiated from iPS cells are cultured for 5 days, and after the cells have sufficiently grown, the neuronal cells colony is physically damaged. Next, dynamic stimulation is applied for 6 days after the damage applied. To apply dynamic stimulation, we use a small piezo electric vibration stage. The vibration condition is set as the amplitude of 60 μm, the frequency of 10 Hz, and the interval stimulation with 16 hours stimulation : 8 hours static in the direction perpendicular to the damaged surface. As a result, it was confirmed that the neurites are reconstructed from the damage by the applied dynamic stimulation.