抄録
This paper presents evaluation of proinflammatory cytokine production amount released by human monocyte-derived macrophages (HMDM) in order to elucidate the effect of wear particles produced by artificial joint on in-vivo immune-function. Polymethylmethacrylate (PMMA) particles were used instead of wear particles for evaluation of effects of particle size on biological responses. The cytotoxicity evaluation test was carried out for evaluating the viability of HMDM which ingested PMMA particles by lactate dehydrogenase activity assay. Also, proinflammatory cytokines production ability of PMMA particles ingested HMDM was evaluated by enzyme-linked immunosorbent assay. As a results, viability of HMDM was approximately 90% or more in the case of PMMA particle size of 0.8, 5.6, 9.6 μm, and was approximately 75% or less in the case of PMMA particle size of 0.16 μm. This result indicates that the HMDM viability was not dependent on the size and the amount of PMMA particles in the case of particle size of 0.8 μm or more. On the other hand, the proinflammatory cytokine production activity of HMDM was dependent on the size of PMMA particles, and 0.8 μm PMMA particle strongly induced the proinflammatory cytokine production.
