オステオネクチンを付与したHAp/コラーゲン複合線維の力学特性に及ぼす作成条件の影響

抄録

The bottom-up-type microstructural optimization of the HAp/collagen composite fibers would enable the creation of the artificial bone mimicking the bone microstructures. In the present study, the effect of the preparation condition on the mechanical properties of HAp/collagen composite fibers was evaluated by means of the micro-tensile tests. Here, the collagen fibers were prepared by the bio-inspired method, where the fibrosis and the cross-linking are generated at the same time. The HAp particles were deposited on the collagen fibers by the biomimetic deposition method, where the collagen fibers are dipped into the pseudo body fluid alternatively. In the present study, the cross-linking agent concentration and the alternative dipping time were varied. In addition, HAp/collagen complex fibers were coated with the adhesive protein before the HAp deposition process. As a result, the coating with the adhesion protein decreased the tensile strength of the HAp/collagen complex fibers prepared under the optimum condition within the range covered in this paper. It was suggested that the adhesive protein would act as the additional cross-linking agent, resulting the excessive cross-linking. Therefore, it can be expected that the coating with the adhesive protein after the insufficient cross-linking could maximize the tensile strength of the HAp/collagen composite fibers with the adhesive protein coating.