2P1-B09 High Speed Evaluation of Cell Stiffness by the Passing Time of Microchannels

Abstract

This paper discusses a real time measurement of the rigidity of the cell in the microchannel. We focus on the passing time of the cell in the microchannel as an index of the rigidity of the cell. Such a real time measurement requires a simple algorithm so that a high speed vision can quickly compute the position of the center of cells. The experimental system is composed of a PDMS microchannel, a phase-contrast microscope, a high speed labeling camera, and PC. From experimental results, we show that a high speed labeling camera can successfully trace the position of a cell in the microchannel.