抄録
Spheroids which cells aggregate and form have far greater functions than individual cells. In particular, hetero-spheroid composed of different cell species such as hepatocytes and endothelial cells express tissue specific function at a high level, which will realize more precise drag screening and biological research. In this study, we propose rapid formation of three-dimensional hetero-spheroid consisting of hepatocytes and endothelial cells using micro-rotation flow. Hepatocytes are covered by collagen gel layers, whose thickness are less than 200 μm, to increase adhesion strength between hepatocytes and endothelial cells. Gel-coated hepatocytes and endothelial cells are collected to form the hetero-spheroids in array by micro-rotational flow in two minutes. This array could control the size of three-dimensional spheroids hydrodynamically with the standard deviations of less than 19 % by varying the cell density of the medium without altering the device geometry.
