抄録
This paper presents a high-throughput methodology to analyze the function of an ATP binding cassette transporter (ABC transporter) by using a microfluidic device that generates a concentration gradient of substrates. In drug development process, determination of the concentration at which the transport of a drug is inhibited by the other one by 50% (IC_<50>) is an important value for safe and effective use of drugs. To determine IC_<50>, substrate transport by ABC transporters at different concentrations must be evaluated. We applied a microfluidic gradient generator, which makes various concentrations of substrates in a single device, for rapid IC_<50> determination. After incubation of ABC transporters at 5 different substrate concentrations in a gradient generator, we succeeded in detecting the difference of substrate transport revealed by the different fluorescence intensity from ABC transporter vesicles.
