Abstract
Recent trends in tissue engineering have aimed at simulating the physiological environment in vitro. So many researchers try to generate much like body tissue constructs by bottom-up approaches in the field of tissue engineering. We proposed new 3D assembly techniques to fabricate a hollow tissue construct using by water transfer printing. And it enables us assembling three-dimensional multilayered tissues to curved surface easily. Also we discussed optimum geometry of development figures. Finally, we succeeded in assembling three-dimensional multilayered tissue into tubular construct by this method. The tissue consists of neonatal normal human dermal fibroblasts (NHDF). These artificial hollow tissues would be used for drug efficiency evaluation and operative training as in vitro simulators.
