The authors have been investigated how to use protists as living micromachines by controlling their behavior using their taxis. Daphnia magna has strong positive phototaxis to the blue light. Therefore, we can control Daphnia's behavior very well by lighting blue light to the aiming angle. In our previous study, we succeeded to make an automatic object manipulation system by using motion controlled Daphnia. We have to prepare special tools for microorganisms to use microorganism that microorganism itself can never do. Daphnia has fairly large body (body length: 3mm) and exoskeleton that enables to glue special tools on Daphnia. Therefore, we could succeed in the previous study to destroy a balloon by a needle installed Daphnia and to make medical injection by using special injector. These tasks, however, were done at just under the water surface and the positions of the height were fixed because we used a wing float to stabilize Daphnia's swimming. Therefore, in this study, we tried to make Daphnia do special works into the water freely. We attached a polypropylene needle on the Daphnia's back as roles of float and special tool. The Daphnia's swimming speed was increased by attaching the needle. We also made a motion control pool in the vertical plane. We could automatically control Daphnia along star-shaped target route on the vertical plane.