This paper presents a local nano-injection method by fluorescence micro-gel-bead and micro-pipette tool based on nano-manipulation under optical micro scope(OM) and electrom micro scope(E-SEM). Micro-gel-bead is made of PEG-DA. It can contain liquid its inside and leak it outside. The top of micro-pipette tool is cut obliquely to fix the micro-gel-bead and it can attract bead by changing air pressure with syringe driver. It is fabricated by focused ion beam(FIB) process. The Caenorhabditis elegans(C.elegans) is important model organism for nerve analysis. The local-nano-injection technique is needed to help nerve regeneration analysis. The proposed system is considered to be important as future nano-sugery system for life innovation.
