We have been developed "Optically Driven Micro-robot" as a novel tool for manipulating a cell and for measuring cellular mechanical properties. In this work, we constructed an innovative system that can measure three dimensional reactive forces from cell and sense posture of the micro-robot with a single normal CCD attached to microscope. The system is realized by installing tapered cylindrical marker triplets to the micro-robot. Compared with conventional 3D imaging system with optical micro-scope, this system has advantages in that it can image and mechanically analyze in 3D from a single plane image acquired from a normal CCD camera. This simple system contributes to the micro analysis useful for cellular biology in where many demands to the micro bio physical measurement.