抄録
We have developed an intracellular delivery system combining an array of needles and microelectrodes to control the functions of single cells at the same time in multiple conditions. In this study, we demonstrated an intracellular delivery using a single glass needle, transparent microelectrodes, and microwell arrays. An array of microwells was fabricated in SU-8 photoresist on a transparent electrode. We applied a DC voltage between an electrode placed in a glass needle and a substrate electrode and generated an electrokinetic flow. A glass needle was inserted into a cell cultured in a microwell and fluorescent DNA was delivered by electrokinetic forces.
