抄録
We have made up a portable device for reverse transcription, the first step in ribonucleic acid (RNA) expression analysis. RNA expression analysis is significant for early detection of diseases. Although quantitative reverse transcription polymerase chain reaction (qRT-PCR) is generally used in the analysis, there are two problems for early detection diagnosis. First, the conventional qRT-PCR devices are too heavy and large for portable use. Also, the conventional devices require a lot of expensive reagents, resulting in high medical cost for the patients. To solve these problems, we aimed to develop a portable micro device for qRT-PCR. In this report, we achieved to perform multiple reverse transcription by 1 ttl by using the prototype device. Basic performance for precise measurement of reverse transcription was verified experimentally.
