がん細胞特異的結合ペプチドスクリーニングのためのマイクロ流体デバイス

抄録

Peptides that can bind to normal cells should be removed in the screening of the tumor specific binding peptide. We developed a microfluidic device for screening of tumor specific binding peptide that has serially-connected chambers for normal cells and for cancer cells. The normal cell is to remove the non-specific binding peptides, and the cancer cell is to capture tumor specific binding peptides. Cell introduction into the chambers without cross-contamination and screening simulation using a fluorescence-labeled antibody are examined. In cell introduction experiment, contaminations of different types of cells were prevented. In the screening simulation, the trapped amount of the non-specific antibodies can be reduced to 46 % in the downstream chamber.