血小板産生のためのバイオリアクターのデザイン

抄録

In this study, we showed a design strategy of a bioreactor to produce platelets (PLTs) from megakaryocytes (MKs) using microfluidic chip. PLTs are generated from MKs by applying fluid force. In conventional study, slit structures have proposed for the improvement of producing PLTs. However, slit structures were difficult to adapt the size variation of MKs. In order to adapt a various size of MKs, we utilized a slope shaped microchannel. The effectiveness of the slope shaped microchannel was evaluated by using microbeads, and the results showed that microbeads were trapped in the slope shaped microchannel corresponding to its size. Finally, we evaluated the relationship between input pressure and flow volume. In the result, the flow volume linearly changed corresponding to input pressure. From these results, we confirmed that the proposed bioreactor will contribute to the on-chip platelet production.