蛍光センサを用いた培養環境の酸素分布モニタリング

抄録

This paper reports the measurement of oxygen distribution inside spheroid using fluorescence microbead sensor. Measurement of oxygen distribution inside the tissue such as spheroid is one of the most important issues for creating high-quality tissue and organ. Fluorescence measurement is suitable since the non-contact measurement is possible. This sensor consists of hydrophilic and biocompatible photo-crosslinkable resin, polyethylene glycol diacrylate, oxygen-sensitive fluorescence indicators, Ru(bpy)₃]Cl₂ and STELLA. Ratio measurement of fluorescence microbead sensor using two indicators for absolute and robust measurement of oxygen against photo-bleaching. The fluorescence microbead sensor was calibrated with oxygen and inserted into the spheroid. A demonstration of measurement of the time-course variation of oxygen concentration inside the spheroid was performed.