自動スフェロイド培養システムによるスフェロイド作製時のピペッティングモーションの最適化の検討

抄録

Our research group established a technique for building three-dimensional cell constructs using only cells. The characteristic of this technique is to use spheroids for cell constructs. Spheroids size were adjusted by number of cells. For automatically create spheroids, we have developed Automatic Spheroid Culture System for spheroids manufacture. However, when the number of cells was small, variation in diameter of spheroids was widely. Therefore, this research was aimed to consider about pipetting motion according to the number of cells, and reproducibility of spheroids manufacture was evaluated. we programed nine patterns of pipetting motions based on current motion. The material were the rabbit mesenchymal stem cells, the numbers of cells/wells as 2 × 10⁴. and the passage number as 7.The experiment enabled us to specify an optimal pipetting motion for the spheroids with numbers of cells/wells as 2 × 10⁴.