シュードカラー処理によるスフェロイド形成特性の可視化

抄録

In tissue engineering, building the cell constructs in vitro have been researched intensively in recent years. Our group established techniques which build three-dimensional cell constructs using spheroids. Then, noninvasive analysis was required. We attempted a noninvasive visualization method of the cell distribution inside spheroids and shape of spheroids using pseudo-color display processing. However, this system cannot be analyzed correctly because of the fixed reference values of the pseudo-color display processing. This study aimed to establish a reference values setting method for the concentration distribution of spheroid pictures and improve the analysis accuracy of the system. Software was improved to automatically set the reference value according to each image. Reference gray-scale value was calculated by using value from the average of the four corners area. Previous software could not be confirmed, however, developed software could be confirmed the cell density reduction inside spheroids from 4th day to 5th day.