誘電泳動力を用いた平面脂質平面膜に対する高速リポソーム導入

抄録

This paper proposed a technique to introduce the membrane protein into the lab-on-chip analysis system having a planar lipid bilayer. The proposed technique utilized a dielectrophoretic force generated by the asymmetric configuration of the solid electrodes on the aqueous buffer separator. By applying the alternating current to the separator and the counter electrode, we manipulated liposomes that could host the membrane proteins on the surface. The key point for the dielectrophoretic manipulation on this system was to fabricate the liposome making the different complex permittivity. In this study, we utilized the centrifugal encapsulation technique with the polyethylene beads and the sucrose inside the liposome. In addition, we analyzed an effective configuration of the droplet separator having the taper-edge on the contour of the micropore. This configuration made a strong interpenetrating dielectrophoretic force at the lipid bilayer, and prompted the fusion of liposome into the lipid bilayer. The separator was fabricated by micromachining technique. Using the separator, we formed the lipid bilayer without evading the solid electrode on the surface. Finally, we elucidated the introduction of the liposome by monitoring with the optical microscopy.