振動誘起流れを用いた細胞スフェロイドの作製と形状評価

抄録

Recently, cell spheroids are attracting large attention in medicine and biology because it can be used for various biomedical applications. In this study, we propose a formation method of cell spheroids on microfluidic chip based on vibration-induced flow. Vibration-induced flow is local flow which is generating around vibrating microobject. Thus, we can form cell spheroids by using flow around vibrating microobjects. We performed formation of cell spheroid by using proposed method. Furthermore, we evaluated the shape of formed cell spheroids. We confirmed that the size of cell spheroid can be changed by changing the center diameter of fabricated microobject. We will evaluate effects of design parameters of microobject and form cell spheroid having little variation in the future.