リファレンス用光環境マイクロセンサを用いた細胞培養環境のpH計測

抄録

This work describes a reference fluorescence microsensor for pH measurement in a cell culture environment without initial pH information. The reference pH microsensor is constituted of a fluorescence pH microsensor made of hydrogel, and polydimethylsiloxane as a barrier layer for hydrogen ion. Reference pH microsensors do not be affected by surrounding pH since PDMS does not pass hydrogen ions. Therefore, pH in the cell culture environment is measured by comparing the fluorescence intensities between fluorescence pH microsensor and reference pH microsensor. In this paper, the fabrication of reference pH microsensor and evaluation of pH dependency of reference pH microsensor were demonstrated.