Simultaneous introduction of various materials into cells based on cell membrane perforation system like infusion bag

Abstract

Our photochemical cell membrane perforation method is new approach for mass cell processing, e.g., cell therapy or regenerative medicine. We are trying to establish a new cell processing system, designed for cell suspension and the suspension is handled within a medical bag like package, so the cell membrane perforation process becomes easy handling and can reduce a risk of contamination. One of the advantages of the system should be simultaneous various materials introduction to cells. In this report, we describe the trial results about the simultaneous introduction of fluorescence marker dye and magnetic nanoparticles into cells using the bag system.