ピエゾ素子駆動レンズを用いた細菌遊泳の3次元追跡

抄録

Because bacterial swimming in the aqueous solution is not affected by gravity, three-dimensional measurement is required to know the swimming trajectory. We tried to track a bacterial cell by detecting the focus deviation of the object moving in the microscope field and by controlling the piezo element installed on the objective lens. Because the cell appears to be black and white depending on the distance from the focal plane in the light microscopy, we detected both cells in the image processing. We succeeded in capturing a bacterial cell that repeats straight swimming and random direction changes in the field of view for more than 10 seconds.