Molecular identification of mosquitoes in Thailand by DNA barcodes

抄録

Mosquitoes are important vectors to transmit vector-borne diseases. Many groups of mosquito are classified as closely related species or species complex. Differentiation by morphology is not described, so molecular techniques have been developed. One of these techniques is DNA barcoding to use as a tool for identify correct species that is important to determine vector capacity and consideration to use an appropriate vector control method. 254 samples (fourteen genera) of mosquitoes in Thailand were collected, extracted DNA and amplified a 650 bp cytochrome oxidase subunit I (COI) by Lep F1 and Lep R1 primers. To differentiate mosquito species, COI sequences were analyzed. The percentage of sequence divergence within species calculated average 1.5%, while sequence divergence between species was average 12%. After phylogenetic analysis, neighbour-joining tree showed many clades that contained mosquito species in various genera, Aedes, Anopheles, Mansonia, Culex, Armigeres and Toxorhynchites. In this study it demonstrated that DNA barcoding could used as a tool for identifying mosquito vectors and used only small piece of tissue for detecting species.