抄録
Phospholipase B (PLB) exists in many microorganisms including pathogenic fungi and is suggested to be an important factor for fungal pathogenicity. For example, PLB has been positively correlated with virulence of Cryptococcus neoformans in murine models of infection. In the opportunity fungus Malassezia species, the phospholipase activity of M. pachydermatis was detected and suggested to play a pathogenic role in the occurrence of skin lesions. Then, in order to investigate the PLB activities of main species of Malassezia, we analyzed and compared the PLB activity of this fungus previously. As a result, a lipid-independent fungus showed the highest PLB activity in all of them. Therefore, to clarify the detailed molecular mechanism of Malassezia PLB, we cloned PLB genes from M. furfur and M. pachydermatis. From the result of sequencing, the amino acid sequence of these genes (MfPLB and MpPLB) have 81% of identity each other and 60-75% of identity with those of PLBs from other fungi. The collaborating researcher: Ms. Linh Thi Thuy Truong
