Abstract
The present authors prepared anthocyanin chloride from the skin of egg-plant by Kuroda's procedure and used it as a sample. The sample was hydrolysed with 20% hydrochloric acid and separated into aglycon and sugar. By paperchromatography, one spot of delphinidin was obtained in the aglycon fraction and one spot of glucose in the sugar fraction. Then by applying the sample to column and thin layer chromatographies, 3 bands were obtained in each cases. The bands were eluted and by comparing the result of paperchromatography and ultraviolet absorption with the systematic research of Bate-Smith, Harbone, and Hayashi and Abe, it was decided that band 1 was p-coumaric acid compound of delphinidin-3, 5-diglucoside, band 2 delphinidin-3, 5-diglucoside, and band 3 delphinidin-3-glucoside, respectively.
