Abstract
The formation of lysinoalanine, N'- (2-amino-2-carboxyethyl) -L-lysine (LAL), during the alkaline treatment of proteins was reported and by the reaction the availability of lysine was reduced. The presence of a large amount of this compound in foods would be toxic.
The determination of this compound is usually performed with amino acid autoanalyser, thin layer chromatograph or gas-liquid chromatograph, but these methods were found to be not always suitable for this determination.
As the micro-determination method, isotachoelectrophoresis has been employed and the application of this method of LAL's assay was investigated.
As a result of the preliminary experiments, it was found that the combination of K cation as a leading cation, and carnitine cation as a terminal cation could lead to the best determination of LAL. By using this system the potential unit value of LAL was obtained, and it was confirmed that this value was completely distinction from those of amino acids. The linear relationship between the zone length and the LAL concentration below 25 nmol/μl was also confirmed and the equation of this linear line was y=0.11χ+0.08.
LAL contents in rat's gastro-intestinal contents measured by this and also by the amino acid autoanalyser method were compared, and the values were well coincided.
