Abstract
As an Experiment I, tocopherols in several tissues of rats in a normal and a vitamin E deficiency groups were temporally analyzed by high performance liquid chromatography, after forced administration p. o. of roughly equal mixture of α-, β-, γ- and δ-tocopherol (m-Toc). Similar to the Experiment I, the Experiment II was made by analyzing tocopherols in adipose tissue and back skin after a forced administration of m-Toc and after consecutive forced administration p. o. of δ-rich mixture of tocopherols (δ-rich-Toc) for 4 days.
1) In general, the peak level of each tocopherol in tissues appeared by the order of small intestine, liver, serum and lung, after administration of m-Toc. It seemed that the uptake and disappearance in these tissues occurred by the order of δ≅γ>β>α.
2) In a single administration of m-Toc, there was no evidence that δ-tocopherol was more readily accumulated in adipose tissue and back skin than the other tocopherols.
3) After the consecutive administration of δ-rich-Toc, the concentration of δ-tocopherol in adipose tissue apd back skin showed to increase remarkably. But such administration of α-tocopherol showed no increase of it in the tissues. It was suggested that the accumulation of δ-tocopherol in these tissues is higher than that of other tocopherols.
4) In the back skin of rats administered δ-rich-Toc, it seemed that δ-tocopherol was mainly present in the fat fraction, while α-tocopherol in the skin tissue.
