Abstract
To establish a detecting method of lipid peroxides in fresh materials, extracting and analyzing procedures were examined by using bovine liver as the material. The solvent containing BHT was used for lipid extraction to prevent autoxidation. After extraction, the lipids was reduced immediately with sodium borohydride and then methylated. The reduced lipids were hydrogenated and derivatized with TMS, and the sample was analyzed with GC-MS. Bovine liver used in this experiment contained 0.012g lipid peroxides in 100g of fresh material. Several kinds of hydroxides which were deduced to have derived from linoleic, arachidonic and docosaenoic acids were detected. The former substances of these hydroxides must be hydroperoxides. This analytical procedure may be useful for peroxide analysis for fresh materials.
