Abstract
This work was undertaken to clarify the effect of pretreatment with dilute HCl solutions on the extractability of true proteins from grasses. After the materials were soaked in 0.5-2N HCl solutions at 22°C for 1-21 days, or at 38°C for 1-24h proteins were extracted from the grasses with 0.05N NaOH solutions. Using this procedure, the extractabilities of grass proteins were markedly increased. The highest values obtained were: red clover 66%, spinach 86%, cabbage 94%, Italian rye grass 79%, leaf of Japanese radish 90%, and so on. The pretreatment at 38°C brought about a marked reduction in the time required for successful protein extraction.
