Abstract
ACE inhibitory substances in black matpe constituted a hydrophilic group with a molecular weight of about 400-800, estimated by gel filtration on a Sephadex G-15 column. These inhibitory substances could not be separated using reverse-phase HPLC, gel filtration or ion exchange columns. The ACE inhibitory substances contained phytic acid, which when extracted had 40% of the total activity. When the ACE inhibitory substances were analyzed by TLC, some ninhydrin-positive spots were observed, whereas the phytic acid fraction contained no ninhydrin-positive spots. These results suggest that the substances are a mixture of phytic acid and ninhydrin-positive material.
