抄録
Many studies have shown that titanium surface roughness or grooved orientation significantly affect the proliferation, migration, differentiation, and protein synthesis of human cultured cells especially of osteoblasts or osteoblast-like cells in vitro. However, few studies have compared the cell behaviors of osteoblasts and other human cells in vitro.
The purpose of the present study was to compare the proliferation and differentiation of human oral cells derived from different origins cultured on titanium plates and glass plates.
Bone cells derived from mandibular fragments, periodontal ligament (PDL) cells from mandibular first premolars, and gingival cells from the maxillary tuberosity region were obtained from the same orthognathic patient. These cells in the 4 th and 7 th passages were seeded (5×103 cells/well) in 24-well plastic culture dishes with or without titanium plates or glass plates and incubation continued for 3, 6, 9, 12, and 15 days. At the end of each incubation period, three types of cells were used for the assays of DNA synthesis and alkaline phosphatase (ALP) activity.
The results were as follows:
1. Three types of cells cultured on plastic dishes, titanium plates, and glass plates produced DNA contents and ALP activity in a time-dependent manner.
2. The level of DNA contents and ALP activity produced from these cells were ranked in the following order, respectively, bone cells≒PDL cells＞gingival cells; bone cells≫PDL cells＞gingival cells.
3. The level of DNA contents and ALP activity produced from any type of cells cultured on different materials were ranked in the following order, plastic dish＞ titanium plate＞ glass plate.
4. Any type of cells in the 4 th passage produced a greater amount of DNA contents and ALP activity as compared to those in the 7 th passage.
These results suggested that bone cells in the 4 th passage cultured on titanium plates as well as plastic dishes show high activity for proliferation and differentiation.
