抄録
An attempt was made to refine platelet rich plasma (PRP) from 28 adults by 4 processes. Due to agglutinations in 7 adults and the trouble of machine in 1 adult, measurements of PRP were done on 20 adults, and the numbers of white blood cells (WBC), red blood cells (RBC), platelets (PLT), and the percentage of PLT collection, were measured.
In all processes, the amount of the measured PRP was 0.3 ml solution. The PRP of the process ① was collected from the demarcation line of the serum component and blood cell component, after serial two-differential centrifugation (the first:2,400 rpm, for 7 minutes; the second:3,000 rpm, for 10 minutes). The PRP of the process ② was collected from the bottom of the second differential centrifugation of the serum component that was collected after the first differential centrifugation. The PRP of the process ③ was collected from the demarcation line, after the second differential centrifugation of the blood component that was collected after the first differential centrifugation of process ②. The PRP of the process ④ was collected from the demarcation line after the second differential centrifugation of the portion of the demarcation line after the first differential centrifugation. In the process ②, the PRP was not refined for rare platelet. In the process ①,③, and ④, the PRP was refined, but the comparison of PRP quantities yielded no significant difference. The average of PLT number was 20.8×104/μl before the differential centrifugation, but it was 98.5×104/μl thereafter. The process ① was recommended for easy operation.
