市販洗口剤と歯磨剤がチタンの表面性状に与える影響

抄録

The purpose of this study was to evaluate the biological effects of dental rinses and dental pastes on titanium-surface properties. Titanium discs were totally covered with commercially available dental rinses or pastes, then the specimens were incubated for two or ten days, respectively. The alterations of color stability and surface roughness before and after the incubation with the dental rinses and pastes were analyzed by a color-difference meter and surface roughness meter, respectively. In addition, the mRNA expressions of CCL2 in human gingival fibroblast cells (hGFB) were investigated when the cells were cultured on the surface of the titanium discs treated with the dental pastes. By biofilm assay using an ATP analyzer, the biofilm formation of Porphyromonas gingivalis on the surface of the titanium disc was examined. There were no significant differences in color stability or surface roughness of the titanium surfaces among all dental rinses. Similarly, dental pastes showed no significant change in color stability or surface roughness. On the other hand, sodium fluoride alone significantly increased color stability, as compared with those of dental pastes examined here ( ANOVA, p＜0.05). The titanium surface after incubation with dental pastes had no effect on the mRNA expression of CCL2 in hGFB and biofilm formation of P. gingivalis. The in vitro data in the present study, taken together, suggested that dental rinses of pH: 4.1～7.4 and dental pastes with and without commercially available fluoride could be used daily by patients treated with dental implants. However, care should be taken in the case of prolonged usage since sodium fluoride might be involved in corrosion of the titanium surface.