Microorganisms utilizing glycerol as a carbon source were isolated from various soils for glycerol/O2bio-fuel cell system. Among 19 locations at the campus of Osaka Prefecture University Collage of Technology and Uchiagegawa flood-control green space in Neyagawa city, the microorganisms isolated from three points (sample numbers 1-2, 1-3 and 1-8) were able to grow in the medium containing 15% glycerol at 37°C and ones from ten locations could proliferate in the same concentration of glycerol at 25°C. In the case of the microorganisms growing at 37°C, they were similar in appearance and seemed to form biofilm. Among ten isolation bacteria at 25°C, three samples (sample number 2-2, 2-3 and 2-5) showed good proliferation in the condition of 15% glycerol medium. The growth profiles of the microorganism which showed the most significant proliferation (sample number 2-5) were also investigated at various glycerol concentrations, resulting that maximum cell density was suppressed at higher glycerol concentration.
