抄録
Ferritin is a class of iron storage proteins. In the present study, two full-length cDNA clone, NtFer1 and NtFer2, encoding ferritin from cDNA library of 3-old tobacco seeding, constructed in the lambda TriplEx2 vector have been isolated and characterized. NtFer1 and NtFer2, were 1214 and 1125 nucleotides long encoding 251 and 259 amino acid residues, respectively. The mature subunit encoded by NtFer1 shares 78.3% and 70.6% homology with two soybean ferritin subunits, while NtFer2 shares 71.7% and 68.4%. Southern blot indicated that NtFer1 was encoded by a two-copy gene and NtFer2 was encoded by a single-copy gene in the tobacco genome. Northern blot analysis showed that NtFer1 and NtFer2 were expressed in leaves as well as in roots, and NtFer1 was well responsive to excess iron then NtFer2. The abundance of NtFer1 mRNA was increased in leaves by exogenous ferritin gene everexpressing, while the abundance of NtFer2 mRNA was stably.
