抄録
In order to monitor global gene expression changes during the nodulation process, we have initiated a SAGE analysis using the model legume, Lotus japonicus. In this study, comparison of gene expression profiles was made using 85,482 and 80,233 tags obtained from uninfected roots and nodule primordia. A statistical test has revealed 408 transcripts whose expression was induced in the nodulation process at the significance level of P <0.01, and 428 were identified as those which may be repressed. The gene identification of tags was done using the public as well as the in-house L. japonicus EST sequences. Extension of the SAGE tags into 3' cDNAs was performed for identification of tags with no matching EST by use of the reverse SAGE (rSAGE) technique. Genomic clones used in the genome sequencing project were PCR-screened to help annotate the novel 3' cDNA sequences.
