抄録
Diatoms are major photosynthetic microorganisms and contribute to nearly 25% the global primary production. The marine diatom Phaeodactylum tricornutum is isolated from brackish water area, where the salinity drastically changes from freshwater levels to seawater levels. Cells were cultured in F/2 enriched artificial seawater containing 0.1, 0.5 and 1.0 M of [NaCl]. Although cells required an acclimation period for several days, they grew well on whole spectrum of [NaCl] examined. Transferring cells grown on each [NaCl] to the distant levels of [NaCl] (i.e., from 0.1 to 1.0 M or from 1.0 to 0.1 M) efficiently arrested cell growth. The activity of Ci uptake was measured with newly developed GC technique and was shown to be enhanced by extra cellular [NaCl]. Given the results, P. tricornutum cells were shown to acclimate to wide range of [NaCl] and to enhance Ci uptake under high [NaCl] condition.
