抄録
Lolium perenne and Poa pratensis are perennial grasses which are used to correct Fe-deficiency of fruit tree in calcareous soil by growing them along the tree. We investigated the response of these grasses to Fe-deficiency, focusing on the secretion of Fe-chelating substances from the roots. Both grasses secreted Fe-chelating substance from the roots in response to Fe-deficiency and the secretion was characterized by a temperature-controlled diurnal rhythm. Three kinds of phytosiderophores were secreted from Lolium perenne (cv. Tove); two of them were 2*-deoxymugineic acid (DMA) and 3-epi-hydroxy-2*-deoxymugineic acid (epiHDMA), while the third one was not identified previously. After purification, this unknown compound was identified as 3-hydroxy-2*-deoxymugineic acid (HDMA) by NMR. On the other hand, DMA, avenic acid A (AV) and an unknown compound were secreted from Poa pratensis (cv. Baron). The purification and identification of this unknown compound are currently being undertaken.
