抄録
Myosin XI is a molecular motor responsible for the cytoplasmic streaming in plant cells. In cultured tobacco BY-2 cells, two subclasses of myosin XI, 170-kDa and 175-kDa myosins, have been identified biochemically and immunocytologically. It has been revealed that the 175-kDa myosin was accumulated in phragmoplasts in mitotic cells. In the present study, we identified the organelle transported by the 175-kDa myosin. In transgenic BY-2 cells expressing GFP-labeled endplasmic reticulum (GFP-ER), the intracellular localization of 175-kDa myosin and ER was studied. Especially in mitotic cells, both were accumulated in the phragmoplasts. This phenomenon was suppressed by laturanculin B, an actin-depolymerizing drug. These results suggested that the 175-kDa myosin is concerned with translocation of ER in the cytoplasmic streaming and in phragmoplast formation.
