抄録
The chloroplast genome of some green plants, green algae, bryophytes and the plastid genome of some red algae and the apicoplast of some apicomplexan parasites have been determined. They are circular double-stranded DNAs, which share a quadripartite structure and characterized by an inverted repeat separated by large and small single-copy regions. Some legume, conifer and red algae are exceptions. We try to determine the chloroplast genome of the cotton, which is the most important natural fiber-producing plant. The Tobacco chloroplast DNA was used as a reference for the design of primers. Primer-walking strategy was adopted. Now almost around 140 kilo-base pairs of the cotton chloroplast DNA sequence were determined, depending on that, it is possible to say the cotton chloroplast DNA follow the rule of the quadripartite chloroplast structure, in addition to the well conserved sequence of the putative genes and genes arrangement.