抄録
Gene expression in Nipponbare and two lines (HW1 and HW5) overexpressing a mutant gene for anthranilate synthase alpha subunit (OASA1D) were evaluated in relation to methyl jasmonate treatment.
Two- factorial ANOVA of microarray data revealed that some (1.7% = 371/21495) genes other than OASA1D were differentially expressed in these three genotypes. Genes involved in the biosynthesis of tryptophan from D-arabino-heptulosonate 7-phosphate were upgregulated by exposure to methyl jasmonate but downstream genes that process tryptophan into other metabolites did not show a similarly coordinated response. This may be a reason for the accumulation of tryptophan in OASA1D lines.
Genes showing significant differences among genotypes were observed in stress signal transduction pathways and in the biosynthesis of other amino acids, hormones, indoles and flavonoids. Pathway analysis of gene expression in transgenic rice has revealed important insights into the effects of genetic transformation and exposure to methyl jasmonate on the rice transcriptome.
